“Iran has no interest to increase tension in the region and it never seeks war with any country, including (the) US,” the president said, quoted by state news agency IRNA.
Rouhani was speaking by phone to his French counterpart Emmanuel Macron, as Tehran and Washington engaged in an escalating war of words following Iran shooting down a US drone last week.
“We have always been committed to regional peace and stability and will make efforts in this respect,” the Iranian president told Macron.
US President Donald Trump said he pulled back from retaliatory strikes on Iran at the last minute, rejecting Tehran’s claim that the aircraft was in its airspace.
But pressure mounted this week with Trump announcing sanctions on Iran’s supreme leader and top officials.
The new measures are the latest against Tehran since Trump pulled out of a landmark nuclear accord between Iran and world powers.
Rouhani blamed the United States for regional tensions and said if Washington had stuck to the deal “we would have witnessed positive developments in the region”.
Iran announced in May it would suspend two of its pledges under the 2015 deal, giving the agreement’s remaining supporters two months to help it circumvent US sanctions.
On Tuesday Tehran’s top security official, Ali Shamkhani said Iran would “forcefully” reduce further commitments from July 7.
This was so “countries who interpreted Iran’s ‘patience’ with weakness and inaction realise that Iran’s answer to the American drone’s violation of its airspace will be no different than its reaction to devious political efforts to limit Iranian people’s absolute rights,” he said, quoted by Fars news agency.
Tehran’s compliance had been based on European promises to support Iran’s economy – which have failed to bear fruit – Rouhani told Macron.
Iran previously announced it would stop observing restrictions on its stocks of enriched uranium and heavy water agreed under the deal.
Tehran accuses Washington of waging economic warfare through its crippling sanctions regime, which on Monday saw Iran’s supreme leader Ayatollah Ali Khamenei blacklisted.
The Trump administration says it is open to talks with Tehran, an offer flatly rejected by Rouhani after the US said it would also sanction his top diplomat.
“At the same time as you call for negotiations, you seek to sanction the foreign minister? It’s obvious that you’re lying,” Rouhani said Tuesday, raising fears of conflict despite both sides saying they are not looking for war.
Washington’s national security advisor, John Bolton, on Tuesday accused Iran of being behind “a long series of unprovoked and unjustifiable attacks.”
The US has blamed Tehran for attacks earlier this month on tankers close to the strategic Strait of Hormuz, through which most Gulf oil exports pass.
Iran has vehemently denied involvement, despite it previously threatening to close the vital waterway.
Trump said Tuesday he is not seeking conflict while warning Iran against hitting US interests.
“Any attack by Iran on anything American will be met with great and overwhelming force. In some areas, overwhelming will mean obliteration,” he tweeted.
Iran and the US broke off diplomatic relations in 1980 over the hostage crisis at the US embassy in Tehran following Iran’s Islamic revolution.
Trump’s comments came after a flurry of diplomatic activity, which saw the UN Security Council issue a unanimous call Monday for dialogue to address the bilateral standoff.
With no sign of calm on Tuesday, China urged “calm and restraint”.